The present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. The effect of operating parameters, impellers speed, operational period, temperature, pH and scale-up were considered.The acetic acid pH: 5.2 increased the leaching of lipoxygenase- 1.The sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has bee...

the present work examines leaching of lipoxygenase-1 from soybean flour employing stirred tank vessel. the effect of operating parameters, impellers speed, operational period, temperature, ph and scale-up were considered.the acetic acid ph: 5.2 increased the leaching of lipoxygenase- 1.the sensitivity of agitator speed and geometrical-scale-up on the enzymeleaching has been conducted. the effec...

Invertase was immobilised on microporous montmorillonite K-10 via adsorption and covalent binding. The immobilised enzymes were tested for sucrose hydrolysis activity in a batch reactor. Km for immobilised systems was greater than free enzyme. The immobilised forms could be reused for 15 continuous cycles without any loss in activity. After 25 cycles, 85% initial activity was retained. A study ...

For effective extraction of glucoamylase from the fermented rice bran by endophytic Aspergillus sp. JAN-25 in solid state fermentation, optimized leaching parameters, 1:6 (w/v) of 0.2 M citrate buffer as leaching agent, soaking time with moldy bran 120 min, leaching temperature 45 oC and leaching pH 4.0 at 150 rpm were found to be the optimum leaching parameters that leached the highest yield o...

Bacterial infections by antibiotic-resistant Staphylococcus aureus strains are among the most common postoperative complications in surgical hernia repair with synthetic mesh. Surface coating of medical devices/implants using antibacterial peptides and enzymes has recently emerged as a potentially effective method for preventing infections. The objective of this study was to evaluate the in vit...

A variety of nano-carriers, nano-particles, nano-fibers, nano-tubes etc. have displayed greater competence to mimic nanoscale environment for the enzyme as compared to microand macro-environments provided by traditional support carriers that have been commonly employed for enzyme immobilization. The porous structured beads (silica, alginate etc.), membranes and fibers (>100 μm) have some inhere...

This chapter described the advancements in development of nanostructured supported material and enzyme immobilization techniques. The functionalized nanomaterials extremely affect inherent mechanical properties provide highest biocompatibility specific nano-environment surrounding enzymes for improving stability, catalytic performance, reaction’s activities. on considerably enhances robustness ...

Extremely simple procedures for the preparation of magnetic chitosan enzyme carriers have been developed and used for the immobilization of lipase and β-galactosidase as model enzymes. In the first case commercially available magnetite microparticles were entrapped in chitosan gel, while in the second case magnetic iron oxides microparticles were synthesized in chitosan matrix from ferrous sulf...

Nitrification inhibitors (Thiourea and nitrification inhibitor “A”) with urea fertilizer were used to enhance the nitrogen efficiency. Nitrification inhibitors and the amount of nitrogen were applied through urea fertilizer to rice seed in Column Lysimeters. The whole experiment was conducted with following treatments such as; urea application as a control at 2gN/10kg (200 kg N/ha), urea applic...

A simple approach is described for preparing poly-(ethylene glycol) hydrogel materials with encapsulated seminapthofluorescein (SNAFL)-organophosphorus hydrolase enzyme conjugates. Direct determination of enzyme-catalyzed neurotoxin hydrolysis is provided by the self-referencing, pH-sensitive dye SNAFL-1, whose emission spectrum changes at lambda = 550 in response to pH. Using spectrofluorimetr...